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You are here:Home » Antibodies » Abs to CD Markers » Anti -Glycophorin A (GYPA, Glycophorin A (includes MN Blood Group, CD235a, CD235a Antigen, Glycophorin A Precursor, GPA, GPErik, GpMiIII, GPSAT, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MNS, MN Sialoglycoprotein, PAS-2, Sialoglycoprotein alpha)

Anti -Glycophorin A (GYPA, Glycophorin A (includes MN Blood Group, CD235a, CD235a Antigen,
Glycophorin A Precursor, GPA, GPErik, GpMiIII, GPSAT, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI,
HGpSta(C), MN, MNS, MN Sialoglycoprotein, PAS-2, Sialoglycoprotein alpha)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. The antibody agglutinates untreated RBCs but it failes to agglutinate papain-treated cells. The antibody HIR2 significantly binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
Catalog #127382
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
AA SequenceMYGKIIFVLLLSAIVSISASSTTGVAMHTSTSSSVTKSYISSQTNDTHKRDTYAATPRAHEVSEISVRTVYPPEEETGERVQLAHHFSEPEITLIIFGVMAGVIGTILLISYGIRRLIKKSPSDVKPLPSPDTDVPLSSVEIENPETSDQ
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2.
PurityPurified by Protein A affinity chromatography.
ImmunogenFull length human GYPA, aa1-150 (AAH13328.1).
SpecificityRecognizes human GYPA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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