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You are here:Home » Antibodies » Abs to Histones » Anti -H2AFX (Histone H2A.x, H2a/x, H2AX)

Anti -H2AFX (Histone H2A.x, H2a/x, H2AX)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IF
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Catalog #127694
ApplicationsSuitable for use in Immunofluorescence and ELISA. Other applications not tested.
Recommended DilutionImmunofluorescence: 10ug/ml
Optimal dilutions to be determined by the researcher.
AA SequenceMSGRGKTGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGHYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTSATVGPKAPSGGKKATQASQEY
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No3F4
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2.
PurityPurified by Protein A affinity chromatography.
ImmunogenFull length recombinant corresponding to aa1-143 from human H2AFX (AAH04915) with GST tag. MW of the GST tag alone is 26kD.
SpecificityRecognizes human H2AFX.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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