HAL

Clone Type

Monoclonal

Host

Mouse

Source

Human

Isotype

IgG2a,k

Clone Number

4F2

Grade

Affinity Purified

Applications

E WB

Crossreactivity

Accession #

NM_002108, NP_002099

Shipping Temp

Blue Ice

Storage Temp

-20°C

L-histidine = urocanate + NH3.

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

Optimal dilutions to be determined by the researcher.

AA Sequence

LAACQGIEFLRPLKTTTPLEKVYDLVRSVVRPWIKDRFMAPDIEAAHRLLLEQKVWEVAAPYIEKYRMEHIPESRPLSPTAFSLQFLHKKSTKIPESEDL

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Partial recombinant corresponding to aa558-658 from human HAL (NP_002099) with GST tag. MW of the GST tag alone is 26kD.

Form

Supplied as a liquid in PBS, pH 7.2.

Purity

Purified by Protein A affinity chromatography.

Specificity

Recognizes human HAL.

References

1. Increased Sensitivity of Histidinemic Mice to UVB Radiation Suggests a Crucial Role of Endogenous Urocanic Acid in Photoprotection. Barresi C, Stremnitzer C, Mlitz V, Kezic S, Kammeyer A, Ghannadan M, Posa-Markaryan K, Selden C, Tschachler E, Eckhart L.J Invest Dermatol. 2010 Aug 5. [Epub ahead of print] 2. Histidase expression in human epidermal keratinocytes: Regulation by differentiation status and all-trans retinoic acid. Eckhart L, Schmidt M, Mildner M, Mlitz V, Abtin A, Ballaun C, Fischer H, Mrass P, Tschachler E.J Dermatol Sci. 2008 Jun;50(3):209-15. Epub 2008 Feb 15.

USBio References

No references available

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127715 Mouse Anti-HAL (Histidine Ammonia-lyase, Histidase, HIS)

Clone Type

Host

Source

Isotype

Clone Number

Grade

Applications

Crossreactivity

Accession #

Shipping Temp

Storage Temp

References

USBio References

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