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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -NUP133 (Nuclear Pore Complex Protein Nup133, Nucleoporin Nup133, Nucleoporin 133kDa, 133kD Nucleoporin, hNUP133, FLJ10814, MGC21133)

Anti -NUP133 (Nuclear Pore Complex Protein Nup133, Nucleoporin Nup133, Nucleoporin 133kDa,
133kD Nucleoporin, hNUP133, FLJ10814, MGC21133)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.
Catalog #130648
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
Optimal dilutions to be determined by the researcher.
AA SequenceLKLEILCKALQRDNWSSSDGKDDPIEVSKDSIFVKILQKLLKDGIQLSEYLPEVKDLLQADQLGSLKSNPYFEFVLKANYEYYVQGQ
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No3E8
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2.
PurityPurified by Protein A affinity chromatography.
ImmunogenPartial recombinant corresponding to aa1069-1156 from human NUP133 (NP_060700) with GST tag. MW of the GST tag alone is 26kD.
SpecificityRecognizes human NUP133.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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