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133550 Mouse Anti-SMARCA2 (Probable Global Transcription Activator SNF2L2, ATP-dependent Helicase SMARCA2, BRG1-associated Factor 190B, BAF190B, Protein Brahma Homolog, hBRM, SNF2-alpha, SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A Member 2, BAF190B, BRM, SNF2A, SNF2L2, FLJ36757, MGC74511)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2a,k
Clone Number
2D12
Grade
Affinity Purified
Applications
E IF WB
Crossreactivity
Hu
Accession #
NM_003070, NP_003061
Shipping Temp
Blue Ice
Storage Temp
-20°C

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Two transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.

Applications
Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications have not tested.
Recommended Dilution
Immunofluorescence: 10ug/ml Optimal dilutions to be determined by the researcher.
AA Sequence
KILLDPNSEEVSEKDAKQIIETAKQDVDDEYSMQYSARGSQSYYTVAHAISERVEKQSALLINGTLKHYQLQGLEWM
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant protein corresponding to aa659-735 from human SMARCA2 with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.4.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human SMARCA2.
Conjugates
Pricing
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