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You are here:Home » Antibodies » Abs to 14-3-3 » Anti -14-3-3

Anti -14-3-3

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH
Belongs to the 14-3-3 family. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein.
Catalog #135867
ApplicationsSuitable for use in RIA, Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:2000
Immunohistochemistry (Paraffin): 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b,k
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
PurityPurified by ammonium sulfate precipitation.
ImmunogenRecombinant corresponding to human 14-3-3 expressed in E.coli.
SpecificityRecognizes human 14-3-3. Species Crossreactivity: Mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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