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You are here:Home » Antibodies » Abs to Actin » Anti -ACTG1 (Actin, Cytoplasmic 2, Gamma-actin, Actin, Cytoplasmic 2, N-terminally Processed, ACTG)

Anti -ACTG1 (Actin, Cytoplasmic 2, Gamma-actin, Actin, Cytoplasmic 2, N-terminally
Processed, ACTG)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH FC
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, is a cytoplasmic actin found in nonmuscle cells.
Catalog #135879
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000
Immunohistochemistry (Paraffin): 5ug/ml
Immunohistochemistry (Frozen): 1:100-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to human ACTG1/Gamma-Actin at N-Terminal nonapeptide of gamma-cytoplasmic actin with an acetylated N-terminal (Ac-EEEIAALVI-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
SpecificityRecognizes human ACTG1/Gamma-Actin. Species Crossreactivity: Chicken, porcine, rabbit, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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