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You are here:Home » Antibodies » Antibodies-Neurofilament » Anti -NEFL (Neurofilament Light Polypeptide, NF-L, NF68, CMT2E, FLJ53642)

Anti -NEFL (Neurofilament Light Polypeptide, NF-L, NF68, CMT2E, FLJ53642)


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Clone Host Grade Applications
Monoclonal Mouse Purified B IH
Neurofilaments are the intermediate filaments found in neuronal cells. They are composed of a combination of subunit proteins which typically includes the neurofilment triplet proteins, NF-L (68kD), NF-M (160kD), and NF-H (200kD). Neurofilaments may also include peripherin, alpha-internexin (NF66), nestin, and vimentin. Antibodies to the various neurofilament subunits are useful biomarkers; the filaments are abundant and biochemically stable. The expression of neurofilament proteins varies during neuronal development and differentiation and between between neuronal cell subtypes, and altered in pathological states. Accumulation of neurofilaments is observed the progression of numerous neurological diseases.
Catalog #136283
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:100-1:1000
Immunohistochemistry (Paraffin): 10ug/ml
Immunohistochemistry (Frozen): 1:25-1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap..
Clone TypeMonoclonal
FormSupplied as a liquid in PBS, 0.09% sodium azide.
ImmunogenSynthetic peptide corresponding to human NEFL/NF-L.
SpecificityRecognizes human NEFL/NF-L.. Species Crossreactivity: Opossum
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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