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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -HDEL

Anti -HDEL

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IF
HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
Catalog #136785
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:50-1:500
Western Blot: 1:100-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2B
Clone No2E7
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to HDEL of yeast Bip at C-terminal.
SpecificityRecognizes yeast HDEL at ~78kD. Species Crossreactivity: Drosophilia, saccharomyce, and plants (barnyard grass, beet, cotton, mung bean, sorghum, wheat).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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