Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Aggrecan Neoepitope (Aggrecan Core Protein, Cartilage-specific Proteoglycan Core Protein, CSPCP, Chondroitin Sulfate Proteoglycan Core Protein 1, Chondroitin Sulfate Proteoglycan 1, AGC1, CSPG1, MSK16, ACAN)

Anti -Aggrecan Neoepitope (Aggrecan Core Protein, Cartilage-specific Proteoglycan Core
Protein, CSPCP, Chondroitin Sulfate Proteoglycan Core Protein 1, Chondroitin Sulfate
Proteoglycan 1, AGC1, CSPG1, MSK16, ACAN)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IF
Catalog #136862
Aggrecan is a member of large, aggregating proteoglycans (also including, versican, brevican and neurocan) found in articular cartilage. Aggrecan is composed of three major domains G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase (ADAMTS).
Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. The C-terminal NITEGE fragment is one of the G1 fragments produced when aggrecan is cleaved with aggrecanase. It is the most abundant fragment found in normal human synovial fluid and articular cartilage, and is the major product produced by aggrecanase cleavage. The NITEGE fragment has also been shown to accumulate rapidly after an acute cartilage injury.
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 1ug/ml
Western Blot: 1ug/ml (band is detected at ~61kD representing the neoepitope sequence (NITEGE) generated by aggrecanase-mediated cleavage at Glu373-Ala374 of aggrecan core proteins)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide, 30% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C G G N (aa387) and I T E G E (aa392) of human aggrecan.
SpecificityRecognizes human Aggrecan. Species Crossreactivity: mouse, rat, bovine, feline and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links