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You are here:Home » Antibodies » Abs to Calcitonin » Anti -CGRP (CALCA, CALC1, Calcitonin Gene-related Peptide 1, Alpha-type CGRP, Calcitonin Gene-related Peptide I)

Anti -CGRP (CALCA, CALC1, Calcitonin Gene-related Peptide 1, Alpha-type CGRP, Calcitonin
Gene-related Peptide I)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum IH IF
Catalog #136882
Calcitonin gene-related peptide (CGRP) is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin.
ApplicationsSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:100-1:200 (Fluorescein), 1:1000-1:2000 (Streptavidin/HRP)
Immunohistochemistry: 10um cryostats. Fixative: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4; 500 mL over ~20-30min. Post Fixation: 1.5h at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationNot determined
FormSupplied as a lyophilized powder, 0.09% sodium azide. Reconstitute with 100ul distilled or deionized H2O.
PuritySerum
ImmunogenSynthetic rat alpha-CGRP coupled to bovine thyroglobulin (BTg) with glutaraldehyde.
SpecificityRecognizes rat CGRP. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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