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You are here:Home » Molecular Biology » Growth Factors-GDNF » GDNF, Recombinant, Human (Glial Cell Line-derived Neurotrophic Factor, hGDNF, Astrocyte-derived Trophic Factor, ATF)

GDNF, Recombinant, Human (Glial Cell Line-derived Neurotrophic Factor, hGDNF,
Astrocyte-derived Trophic Factor, ATF)

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Specifications

GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Catalog #136898
SourceRecombinant corresponding to human GDNF, expressed in E.coli.
Molecular Weight~30.36kD
Biological ActivityhGDNF is fully biologically active when compared to a standard. The ED50, calculated by the dose-dependent dopamine uptake in rat mesencephalic cultures, was found to be 4-8ng/ml.
AA SequenceMSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ
ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI
Endotoxin ~0.01ng/ug or ~0.1EU/ug (LAL)
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~30.3kD
SourceE.coli
Purity~98% (RP-HPLC and SDS-PAGE)
Concentration~0.1mg/ml after reconstitution
FormSupplied as a lyophilized powder from 10mM sodium citrate, 100mM sodium chloride, pH 4.0. Reconstitute with sterile H2O to 100ug/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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