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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Ki67 (Antigen KI-67, MKI67)

Anti -Ki67 (Antigen KI-67, MKI67)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IH IC IF
Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki67 index and histologically low-grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
Catalog #136914
ApplicationsSuitable for use in Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin fixed paraffin embedded): 1:20
Immunohistochemistry (Frozen): 1:20
Immunocytochemistry: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to 62 base pair region of the human Ki-67 antigen.
SpecificityRecognizes human Ki67. Species Crossreactivity: mouse and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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