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137033 CPSF4, Recombinant, Human, aa1-244, His-Tag (Cleavage and Polyadenylation Specific Factor 4/30kD, CPSF30, NAR, NEB1) CAS:

Specifications
References
Grade
Purified
Molecular Weight
29.9
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30kD subunit of CPSF4 an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus- infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30kD protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs.

Source
Recombinant protein corresponding to human CPSF4, fused to His-tag at N-terminal expressed in E.coli.
Molecular Weight
~29.9kD (267aa)
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMQEIIAS VDHIKFDLEI AVEQQLGAQP LPFPGMDKSG AAVCEFFLKA ACGKGGMCPF RHISGEKTVV CKHWLRGLCK KGDQCEFLHE YDMTKMPECY FYSKFGECSN KECPFLHIDP ESKIKDCPWY DRGFCKHGPL CRHRHTRRVI CVNYLVGFCP EGPSCKFMHP RFELPMGTTE QPPLPQQTQP PAKQRTPQVI GVMQSQNSSA GNRGPRPLEQ VTCYKCGEKG HYANRCTKGH LAFLSGQ
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥85% (SDS-PAGE)
Concentration
~1mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.4M urea, 10% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Jenny,A., et al. (1994) Mol. Cell. Biol. 14(12):8183-8190. 2. Barabino,S.M., et al. (1997) Nephrol Genes Dev. 11(13):1703-1716.
USBio References
No references available
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