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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -ADAP (Adhesion and Degranulation-promoting Adaptor Protein, SLAP-130, FYB)

Anti -ADAP (Adhesion and Degranulation-promoting Adaptor Protein, SLAP-130, FYB)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
ADAP (adhesion and degranulation-promoting adaptor protein/SLAP-130/Fyb) is an SH3 domain-containing adaptor protein expressed by T cells, NK cells, and myeloid cells (1,2). There are two isoforms of ADAP with predicted molecular weights of 85 kDa and 90 kDa, but observed molecular weights of 120 kDa and 130 kDa (1-3). ADAP was identified as an adaptor protein that interacts with SLP-76 following T cell receptor (TCR) stimulation and was subsequently found to be important for several aspects of T cell activation (1,2). For example, ADAP is required for integrin-dependent clustering, signaling, and adhesion (4,5). In addition, ADAP interacts with CARMA1 and facilitates assembly of the CARMA1-Bcl10-MALT1 complex important for NF-kB activation downstream of TCR activation (6). Finally, following binding of a T cell to an antigen presenting cell, ADAP forms a ring at the immunological synapse that recruits dynein to enable microtubule-organizing center polarization (7).
Catalog #137405
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to residues surrounding Pro731 of human ADAP.
SpecificityRecognizes endogenous levels of total human ADAP. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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