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137420 Rabbit Anti-Caldesmon-1 (CAD, CALD1)

Specifications
References
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Swiss Prot
Q05682
Isotype
IgG
Clone Number
13G23(D5C8D)
Grade
Purified
Applications
IF IP WB
Crossreactivity
Hu Mk Mo Rt
Gene ID
800
Shipping Temp
Blue Ice
Storage Temp
-20°C

Caldesmon-1 is an actin filament stabilizing protein involved in the regulation of cell contraction. Binding of caldesmon-1 to actin is weakened by phosphorylation and by calmodulin in the presence of calcium. Caldesmon-1 is encoded by a single gene, which is spliced to generate a widely distributed low molecular weight form and a smooth muscle specific high molecular weight form (1,2). Caldesmon-1 is phosphorylated by the cyclin dependent kinase cdc2 and Erk1/2 MAP kinase, both of which prevent the activity of caldesmon-1 (3-5). Phosphorylation of caldesmon-1 by cdc2 is required for passage of cells through mitosis (6). Phosphorylation by Erk1/2 is important in regulating smooth muscle contraction (7). Caldesmon-1 activity may play a role in the formation of podosomes, adhesion complexes associated with the secretion of matrix metalloproteases, invasion, and metastasis (reviewed in 5).

Applications
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Immunofluorescence(Immunocytochemistry): 1:200 Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues near the C-terminal domain of human caldesmon-1.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified.
Specificity
Recognizes endogenous levels of total human Caldesmon-1. Predicted to cross-react with both smooth muscle and nonmuscle isoforms. Species Crossreactivity: mouse, rat, monkey.
References
1. Hayashi, K. et al. (1992) Proc. Natl. Acad. Sci. USA 89, 12122-12126. 2. Humphrey, M.B. et al. (1992) Gene 112, 197-204. 3. Yamashiro, S. et al. (1991) Nature 349, 169-172. 4. Mak, A.S. et al. (1991) J. Biol. Chem. 266, 6678-6681. 5. Hai, C.M. and Gu, Z. (2006) Eur. J. Cell Biol. 85, 305-309. 6. Yamashiro, S. et al. (2001) Mol. Biol. Cell 12, 239-250. 7. Hedges, J.C. et al. (2000) Am. J. Physiol. Cell Physiol. 278, C718-C7126.
USBio References
No references available
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