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Specifications

Clone Host Grade Applications Monoclonal Rabbit Purified B IP IF

Caldesmon-1 is an actin filament stabilizing protein involved in the regulation of cell contraction. Binding of caldesmon-1 to actin is weakened by phosphorylation and by calmodulin in the presence of calcium. Caldesmon-1 is encoded by a single gene, which is spliced to generate a widely distributed low molecular weight form and a smooth muscle specific high molecular weight form (1,2). Caldesmon-1 is phosphorylated by the cyclin dependent kinase cdc2 and Erk1/2 MAP kinase, both of which prevent the activity of caldesmon-1 (3-5). Phosphorylation of caldesmon-1 by cdc2 is required for passage of cells through mitosis (6). Phosphorylation by Erk1/2 is important in regulating smooth muscle contraction (7). Caldesmon-1 activity may play a role in the formation of podosomes, adhesion complexes associated with the secretion of matrix metalloproteases, invasion, and metastasis (reviewed in 5).
Catalog #	137420
Applications	Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Immunofluorescence(Immunocytochemistry): 1:200
	Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
	Immunoprecipitation: 1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Monoclonal
Isotype	IgG
Clone No	13G23
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide.
Purity	Purified.
Immunogen	Synthetic peptide corresponding to residues near the C-terminal domain of human caldesmon-1.
Specificity	Recognizes endogenous levels of total human Caldesmon-1. Predicted to cross-react with both smooth muscle and nonmuscle isoforms. Species Crossreactivity: mouse, rat, monkey.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.