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You are here:Home » Antibodies » Abs to Topoisomerases » Anti -DNA Topoisomerase II (Topoisomerase II-alpha )

Anti -DNA Topoisomerase II (Topoisomerase II-alpha )

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B IH FC IF
DNA topoisomerase I and II are nuclear enzymes, and type II consists of two highly homologous isoforms: topoisomerase IIa and IIb. These enzymes regulate the topology of DNA, maintain genomic integrity, and are essential for processes such as DNA replication, recombination, transcription, and chromosome segregation by allowing DNA strands to pass through each other (1). Topoisomerase I nicks and rejoins one strand of the duplex DNA, while topoisomerase II transiently breaks and closes double-stranded DNA (2). Topoisomerases are very susceptible to various stresses. Acidic pH or oxidative stress can convert topoisomerases to DNA-breaking nucleases, causing genomic instability and cell death. DNA-damaging topoisomerase targeting drugs (e.g., etoposide) also convert topoisomerases to nucleases, and the enzyme is usually trapped as an intermediate, covalently bound to the 5+ end of the cleaved DNA strand(s). Research studies have shown that this intermediate leads to genomic instability and cell death, and thus, agents that target topoisomerases are highly sought after cancer chemotherapeutic drugs (3). Ca2+-regulated phosphorylation of topoisomerase IIa at Ser1106 modulates the activity of this enzyme and its sensitivity to targeting drugs (4).
Catalog #137430
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:1600
Flow Cytometry: 1:100
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry(Paraffin): 1:400
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No13G28
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide.
PurityPurified.
ImmunogenSynthetic peptide corresponding to residues near the C-terminal of human topoisomerase IIa.
SpecificityRecognizes endogenous levels of total human Topoisomerase IIa. Species Crossreactivity: monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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