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137431 Rabbit Anti-EIF1 (Eukaryotic Translation Initiation Factor 1)

Specifications
References
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Swiss Prot
P41567
Isotype
IgG
Clone Number
13G29(D7G3L)
Grade
Purified
Applications
IP WB
Crossreactivity
Hu Mk Mo Rt
Gene ID
10209
Shipping Temp
Blue Ice
Storage Temp
-20°C

Eukaryotic translation initiation factor 1 (eIF1) was first purified as a factor stimulating binding of Met-tRNA and mRNA to the ribosome (1,2). eIF1 is essential for growth in yeast and two classes of mutations in yeast eIF1 indicate a role for this protein in ensuring accurate translation initiation site selection (3). It has been demonstrated that eIF1 expression is stress-inducible, suggesting that modulation of translation initiation occurs during cellular stress (4).

Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues near the N-terminal domain of human eIF1. Species Sequence Homology: canine
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified.
Specificity
Recognizes endogenous levels of total human eIF1. Predicted to react with eIF1B. Species Crossreactivity: mouse, rat, monkey.
References
1. Schreier, M.H. et al. (1977) J Mol Biol 116, 727-53. 2. Trachsel, H. et al. (1977) J Mol Biol 116, 755-67. 3. Yoon, H.J. and Donahue, T.F. (1992) Mol Cell Biol 12, 248-60. 4. Sheikh, M.S. et al. (1999) J Biol Chem 274, 16487-93.
USBio References
No references available
Conjugates
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