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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -EIF1 (Eukaryotic Translation Initiation Factor 1)

Anti -EIF1 (Eukaryotic Translation Initiation Factor 1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B IP
Eukaryotic translation initiation factor 1 (eIF1) was first purified as a factor stimulating binding of Met-tRNA and mRNA to the ribosome (1,2). eIF1 is essential for growth in yeast and two classes of mutations in yeast eIF1 indicate a role for this protein in ensuring accurate translation initiation site selection (3). It has been demonstrated that eIF1 expression is stress-inducible, suggesting that modulation of translation initiation occurs during cellular stress (4).
Catalog #137431
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No13G29
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide
PurityPurified.
ImmunogenSynthetic peptide corresponding to residues near the N-terminal domain of human eIF1. Species Sequence Homology: canine
SpecificityRecognizes endogenous levels of total human eIF1. Predicted to react with eIF1B. Species Crossreactivity: mouse, rat, monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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