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Anti -EIF1 (Eukaryotic Translation Initiation Factor 1)
Anti -EIF1 (Eukaryotic Translation Initiation Factor 1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Purified |
B IP |
|
| Eukaryotic translation initiation factor 1 (eIF1) was first purified as a factor stimulating binding of Met-tRNA and mRNA to the ribosome (1,2). eIF1 is essential for growth in yeast and two classes of mutations in yeast eIF1 indicate a role for this protein in ensuring accurate translation initiation site selection (3). It has been demonstrated that eIF1 expression is stress-inducible, suggesting that modulation of translation initiation occurs during cellular stress (4). | | | Catalog # | 137431 | | Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. | | Immunoprecipitation: 1:100 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 13G29 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide | | Purity | Purified. | | Immunogen | Synthetic peptide corresponding to residues near the N-terminal domain of human eIF1. Species Sequence Homology: canine | | Specificity | Recognizes endogenous levels of total human eIF1. Predicted to react with eIF1B. Species Crossreactivity: mouse, rat, monkey. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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