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You are here:Home » Molecular Biology » Growth Factors-Interleukin » IL-6, Recombinant, Mouse, His-Tag (Interleukin-6, His6Interleukin-6, His6IL-6) (Carrier free)

IL-6, Recombinant, Mouse, His-Tag (Interleukin-6, His6Interleukin-6, His6IL-6) (Carrier
free)

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Specifications

IL-6 is a potent inducer of the acute phase response and is produced by T cells, macrophages, fibroblasts, endothelial, and other cells (1,2). IL-6 induces proliferation and differentiation and acts on B cells, T cells, thymocytes, among others. IL-6 in concert with TGF-beta is important for developing Th17 responses. IL-6 binds to IL-6Ralpha and through this association induces gp130 homodimerization (1). gp130 homodimerization triggers the Jak/Stat cascade and the SHP2/MAPK (Erk) cascade (1,3,4). IL-6 also forms a complex with an IL-6Ralpha splice variant that is non-membrane associated (3). This IL-6/soluble IL-6Ralpha complex can activate the gp130 signaling pathway on cells that express gp130, but not IL-6Ralpha (3). Research studies have shown that IL-6 may contribute to metastatic breast cancer by increasing expression of proangiogenic VEGF (5).
Catalog #137451
SourceRecombinant protein corresponding to mouse His6IL-6 at N-terminal, fused to His-tag expressed in human 293 cells.
Molecular Weight~34-36kD
Biological ActivityDetermined in a B9 cell proliferation assay. The ED50 of each lot is 0.5-5pg/ml.
Endotoxin<0.01ng/1ug
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~34-36kD
Purity~98% (SDS-PAGE)
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in sterile PBS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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