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You are here:Home » Molecular Biology » Growth Factors-LIF » LIF, Recombinant, Human (Leukemia Inhibitory Factor, hLIF)

LIF, Recombinant, Human (Leukemia Inhibitory Factor, hLIF)

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Specifications

Leukemia Inhibitory Factor (LIF) is a 20 kDa pleiotrophic factor belonging to the IL-6 superfamily of cytokines (1). LIF is expressed in a number of tissues and cell types. The LIF receptor is a heterodimer comprised of LIF-R (gp190) and gp130, a common signal transducer for IL-6-type cytokines (1). Depending on cell type and context, LIF/LIF-R can activate Erk, PI3K, and Jak1/Stat1/3 pathways (1,2). LIF has a diverse array of biological activities. Murine embryonic stem cells are dependent on LIF for pluripotency and self-renewal in vitro (1). Exercise- induced LIF secretion in muscle induces myoblast proliferation, suggesting that LIF may play a role in exercise-induced muscle hypertrophy (2). LIF also negatively regulates Th2 and Th17 cell differentiation (3,4).
Catalog #137462
SourceRecombinant protein corresponding to human LIF, expressed in E. coli.
Molecular Weight~20kD
Biological ActivityDetermined in a TF-1 cell proliferation assay. The ED50 of each lot is 0.2-1.5ng/ml.
Endotoxin<0.01ng/1ug
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~20kD
SourceE. coli
Purity~98% (SDS-PAGE)
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, pH 7.2, 20ug BSA/ug recombinant LIF. Reconstitute in sterile PBS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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