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You are here:Home » Antibodies » Abs to IgG Kappa » Anti -Kappa Light Chain (HRP)

Anti -Kappa Light Chain (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH IC
There are two types of light chain in mammals, the kappa chain; encoded by the immunoglobulin kappa locus on chromosome 2, and the lambda chain; encoded by the immunoglobulin lambda locus on chromosome 22. Antibodies are produced by B lymphocytes, each expressing only one class of light chain. Once set, light chain class remains fixed for the life of the B lymphocyte.
Catalog #138063
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:100,000
Western Blot (colorimetric): 1:1000-1:10,000,
Western Blot (chemiluminescent): 1:1000-1:30,000
Immunohistochemistry: 1:200-1:500
Immunocytochemistry: 1:200-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.2% BSA, 0.1% Pro-Clean 400. Labeled with horseradish peroxidase (HRP).
PurityPurified by immunoaffinity chromatography.
ImmunogenMouse kappa light chain
SpecificityRecognizes mouse kappa light chain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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