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138521-AF Mouse Anti-CD56 (Neural Cell Adhesion Molecule, NCAM)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Swiss Prot
P13591
Isotype
IgG1,k
Clone Number
123C3.D5
Grade
Affinity Purified
Applications
E FC IF IHC
Crossreactivity
Hu
Accession #
NP_000606
Gene ID
4684
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

CD56, a 175-220KD glycoprotein, is a member of the Ig super family. It is expressed as three major isoforms and consists of five Ig-like domains and two Fibronectin-type III domains in the extracellular region. The 135kD isoform is the basic molecule which is glycosylated or sialylated to produce the mature species. CD56 is widely expressed in nervous system, on NK cells and a specific set of Tcells. CD56+ NK cells and Tcells are unique in their ability to mediate cell-mediated cytotoxicity against certain tumor cell targets without MHC restriction. Other physiological functions of CD56 include mediating cell adhesion through homophilic and heterophilic interaction and activating intracellular signaling pathways resulting in neutrite extension and fasciculation, migration and synapses formation in brain. CD56 is also vital for neuronal development and plasticity in adult brain. It is used as a tumor marker in various cancers such as NK lymphomas and Merkel cell carcinoma. NCAM is expressed on most neuroectodermal derived lines, tissues, and neoplasms such as retinoblastoma, medulloblastoma, astrocytoma, and neuroblastoma. It is also expressed on some mesodermally derived tumors such as rhabdomyosarcoma and also on natural killer cells.

Applications
Suitable for use in ELISA, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (Paraffin): Requires heating tissue sections in 10mM Tris, 1mM EDTA pH 9.0, for 45 minutes at 95°C followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher.
Positive Control
Cerebellum, Pancreas, Neuroblastoma
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Membrane preparation of a small cell lung carcinoma. Cellular Localization: Cell surface
Form
Supplied as a liquid in PBS. Also available with BSA and azide, see 138521.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes CD56. Species Crossreactivity: human.
References
1. Gerardy-Schahn R; et. al. Int Journal of Cancer. Supplement, 1994, 8:38-42. 2. Michalides R; et. al. International Journal of Cancer. Supplement, 1994, 8:34-7. 3. Schol DJ; et. al. Int J Cancer. Supplement, 1988, 2:34-40.
USBio References
No references available
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