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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Cytokeratin 17 (Cytokeratin-17, CK 17, CK17, CK-17, Keratin 17, Keratin-17, Keratin Type I Cytoskeletal 17) (FITC)

Anti -Cytokeratin 17 (Cytokeratin-17, CK 17, CK17, CK-17, Keratin 17, Keratin-17, Keratin
Type I Cytoskeletal 17) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC IF
Cytokeratin 17 (CK17) is a member of the Cytokeratin subfamily of intermediate filament proteins (IFP's). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. CK17 is expressed in the nail bed, hair follicle, sebaceous glands and other epidermal appendages. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glands such as mammary, sweat and salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered as "epithelial stem cell" marker because CK17 Ab marks basal cell differentiation. CK17 can be useful when included in a panel of antibodies against TTF-1, napsin A, CK5&6, p63, and SOX-2 for diagnostic differentiation between lung adenocarcinoma (LADC) and lung squamous cell carcinoma (SCLC), especially for poorly-differentiated lung carcinoma. CK17 is expressed in SCLC much higher than in LADC. In breast carcinomas, approximately 20% of patients show no expression of ER, PR and Her2, which are defined as triple negative tumor. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype.
Catalog #138536-FITC
ApplicationsSuitable for use in FLISA, Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-1ug/ml
Flow Cytometry: 0.5-1ug/10e6 cells
Immunofluorescence: 1-2ug/ml
Immunoprecipitation: 1-2ug/500ug protein lysate
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT
Optimal dilutions to be determined by the researcher.
Cellular LocalizationCytoplasmic
Positive ControlSkin
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone NoE3, Ks17.E3
HostMouse
SourceRat
ConcentrationAs reported
FormSupplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Conjugated to Fluorescein isothiocyanate (FITC).
PurityPurified by Protein G affinity chromatography.
ImmunogenThe cytoskeletal fraction of rat colon epithelium.
SpecificityRecognizes rat cytokeratin 17. Species Crossreactivity: human, canine, goat and porcine. Reacts with basal cells in complex epithelia and reacts strongly with SCCs.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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