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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Cytokeratin, HMW (High Molecular Weight Keratin) (AP)

Anti -Cytokeratin, HMW (High Molecular Weight Keratin) (AP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH FC IF
This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kD (CK1); 64kD (CK3); 59kD (CK4); 58kD (CK5); 56kD (CK6); 52kD (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kD. Monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kD keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Catalog #138537-AP
ApplicationsSuitable for use in ELISA, Immunofluorescence, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.5-1ug/10e6 cells
Western Blot: 0.5-1ug/ml
Immunofluorescence: 1-2ug/ml
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT
Optimal dilutions to be determined by the researcher.
Positive ControlSkin, Adenocarcinomas
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Note Applications are based on unconjugated antibody.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone NoAE-3
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Conjugated at Alkaline Phosphatase (AP).
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman epidermal keratin
SpecificityRecognizes human cytokeratin. Species Crossreactivity: monkey, bovine, canine, rabbit, mouse, rat and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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