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You are here:Home » Antibodies » Antibodies-Keratin, Cytokeratin » Anti -Cytokeratin, pan (Pan Keratin) (BSA & Azide Free)

Anti -Cytokeratin, pan (Pan Keratin) (BSA & Azide Free)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC IF
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5 , CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing ce lls in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Catalog #138538-AF
ApplicationsSuitable for use in Western Blot, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-1ug/ml for 2 hours at RT
Flow Cytometry: 0.5-1ug/10e6 cells
Immunofluorescence: 1-2ug/ml
Immunohistochemistry (Formalin fixed): 0.25-0.5ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissues sections in 10mM sodium citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlSkin, Adeno- or Squamous carcinomas
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone NoAE-1/AE-3
FormSupplied as a liquid in PBS. Also available with BSA & azide, see 138538.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman epidermal keratin
SpecificityRecognizes human pan cytokeratin. Species Crossreactivity: monkey, bovine, canine, rabbit, mouse, rat, chicken
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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