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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Cytokeratin, pan (Pan Keratin) (AP)

Anti -Cytokeratin, pan (Pan Keratin) (AP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC IF
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5 , CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing ce lls in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Catalog #138538-AP
ApplicationsSuitable for use in Western Blot, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-1ug/ml for 2h @ RT
Flow Cytometry: 0.5-1ug/10e6 cells
Immunofluorescence: 1-2ug/ml
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT
Optimal dilutions to be determined by the researcher.
Positive ControlSkin, Adeno- or Squamous carcinomas
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Note Applications are based on unconjugated antibody.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone NoAE-1/AE-3
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Conjugated at Alkaline Phosphatase (AP).
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman epidermal keratin
SpecificityRecognizes human pan cytokeratin. Species Crossreactivity: monkey, bovine, canine, rabbit, mouse, rat and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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