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You are here:Home » Antibodies » Abs to Transmembrane Proteins » Anti -DOG1 (Discovered on Gastrointestinal Stromal Tumors Protein 1, GITS)

Anti -DOG1 (Discovered on Gastrointestinal Stromal Tumors Protein 1, GITS)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse B IP IH FC IF
Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GIST's), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8% of scorable GIST's, including all c-kit negative GIST's. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha (PDGFR alpha) mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GIST's is nearly identical: 94.4% vs. 94.7%.
Catalog #138539
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 5-10ul/10e6 cells
Western Blot: 1:100-1:200
Immunofluorescence: 1:50-1:100
Immunoprecipitation: 5-10ul/500ug protein lysate
Immunohistochemistry (Formalin fixed paraffin embedded): 1:100-1:200/30min @ RT
Optimal dilutions to be determined by the researcher.
Positive ControlGastrointestinal Stromal Tumor (GIST) or testicular germ cell tumor. Melanocytes in the basal layer of the epidermis and mast cells in the derm is of normal skin.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone NoDG1/447
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid, 0.05% sodium azide.
ImmunogenRecombinant protein corresponding to human DOG1.
SpecificityRecognizes human DOG1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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