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You are here:Home » Antibodies » Antibodies-Mucin » Anti -Epithelial Membrane Antigen (MUC-1, Mucin-1, CA15-3, CA27-29, CD227, DF3, EMA, EMA E29, H23AG, KL-6, MAM6, PEM, PEMT, PUM)

Anti -Epithelial Membrane Antigen (MUC-1, Mucin-1, CA15-3, CA27-29, CD227, DF3, EMA, EMA
E29, H23AG, KL-6, MAM6, PEM, PEMT, PUM)


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Clone Host Grade Applications
Monoclonal Mouse Supernatant B IP IH FC IF
In Western blotting, it recognizes proteins in MW range of 265-400kD, identified as different glycoforms of EMA. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit and the C-terminal beta subunit. The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Catalog #138546
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 5-10ul/10e6 cells
Immunofluorescence: 1:50-1:100
Western Blot: 1:100-1:200
Immunoprecipitation: 5-10ul/500ug protein lysate
Immunohistochemistry (Formalin fixed paraffin embedded): 1:100-1:200/30min @ RT
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone NoE29
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
ImmunogenDelipidated extract of human milk fat globule membranes
SpecificityRecognizes human EMA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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