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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor (ER)

Anti -Estrogen Receptor (ER)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
This monoclonal antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.
Catalog #138548
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.5-1ug/10e6 cells
Immunohistochemistry (Formalin fixed paraffin embedded): 10-20min followed by cooling @ RT for 20min
Western Blot: 0.5-1ug/ml for 2h @ RT
Optimal dilutions to be determined by the researcher.
Positive ControlMCF-7 Cells or Breast Cancers
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone NoER506
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138548AF.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa2-185 from human Estrogen Receptor alpha.
SpecificityRecognizes human Estrogen Receptor alpha.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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