Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Western Blot: 0.5-1ug/ml Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (FFPE): 0.25-0.5ug/ml/30min at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilutions to be determined by the researcher.

Brain or Astrocytoma

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

GFAP isolated from pig spinal cord (NP_001124491; P14136; P03995; P47819).

Supplied as a liquid in PBS. Also available with BSA and azide, see 138553.

Purified by Protein G affinity chromatography.

Recognizes GFAP at ~50kD. Species Crossreactivity: human, mouse, rat, bovine, porcine, rabbit, chicken. Does not crossreact with other intermediate filament proteins.