Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to IgG Kappa » Anti -Kappa Chain (B Cells) (FITC)

Anti -Kappa Chain (B Cells) (FITC)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC
This monoclonal antibody is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Catalog #138557-FITC
ApplicationsSuitable for use in FLISA, Western Blot, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.5-2ug/10e6 cells
Western Blot: 0.5-1ug/ml
Immunoprecipitation: 1-2ug/500ug protein lysate
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0. 5-1ug/ml/30min @ RT
Optimal dilutions to be determined by the researcher.
Positive Control293 T, Raji or hPBL cells. Tonsil or Spleen
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Note Applications are based on unconjugated antibody.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone NoL1C1
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Conjugated to Fluorescein isothiocyanate (FITC).
PurityPurified by Protein G affinity chromatography.
ImmunogenB lymphoma cells
SpecificityRecognizes human kappa. Does not react with rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links