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You are here:Home » Antibodies » Abs to IgG Lambda » Anti -Lambda Chain (B cells)

Anti -Lambda Chain (B cells)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH FC IF
This monoclonal antibody is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Catalog #138563
ApplicationsSuitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 1-2ug/ml
Flow Cytometry: 0.5-1ug/10e6 cells
Western Blot: 0.5-1ug/ml
Immunoprecipitation: 1-2ug/500ug protein lysate
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT
Optimal dilutions to be determined by the researcher.
Positive Control293T, Raji or hPBL cells. Tonsil or Spleen
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone NoICO-106
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138563AF.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified human IgG
SpecificityRecognizes human lambda.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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