Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to IgG Lambda » Anti -Lambda Light Chain

Anti -Lambda Light Chain

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH FC IF
This monoclonal antibody is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Catalog #138565
ApplicationsSuitable for use in ELISA, Flow Cytometry, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-1ug/ml
Flow Cytometry: 0.5-1ug/10e6 cells
Immunofluorescence: 1-2ug/ml
Immunoprecipitation: 1-2ug/500ug protein lysate
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT
Optimal dilutions to be determined by the researcher.
Cellular LocalizationCell Surface, cytoplasmic and secreted
Positive Control293 T, Raji or hPBL cells. Tonsil or Spleen
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone NoHP6054
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138565AF.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified human IgG myeloma proteins covalently coupled to polyaminostyren microbeads.
SpecificityRecognizes human lambda light chain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links