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You are here:Home » Antibodies » Abs to Mucin » Anti -MUC-1 (EMA, Epithelial Membrane Antigen Mucin-1 )

Anti -MUC-1 (EMA, Epithelial Membrane Antigen Mucin-1 )

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH FC IF
In Western blotting, it recognizes proteins in MW range of 265-400kD, identified as different glycoforms of EMA. This monoclonal antibody reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylation s and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Catalog #138571
ApplicationsSuitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.5-1ug/10e6 cells
Western Blot: 0.5-1ug/ml
Immunofluorescence: 1-2ug/ml
Immunoprecipitation: 1-2ug/500ug protein lysate
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT
Optimal dilutions to be determined by the researcher.
Positive ControlMCF-7 or MDA-231 cells. Breast or colon carcinoma.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone NoGP1.4
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138571AF.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman milk fat globule membrane
SpecificityRecognizes human EMA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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