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138571 Mouse Anti-MUC-1 (EMA, Epithelial Membrane Antigen Mucin-1 )

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P15941
Isotype
IgG1,k
Clone Number
GP1.4
Grade
Affinity Purified
Applications
E FC IF IHC IP WB
Crossreactivity
Hu
Accession #
NP_001018016
Gene ID
4582
Shipping Temp
Blue Ice
Storage Temp
-20°C

In Western blotting, it recognizes proteins in MW range of 265-400kD, identified as different glycoforms of EMA. This monoclonal antibody reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylation s and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Applications
Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher.
Positive Control
MCF-7 or MDA-231 cells. Breast or colon carcinoma.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human milk fat globule membrane
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138571-AF.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human EMA.
References
1. Stanley CM, Phillips TE. Am J Physiol. 1999 Jul;277 (1 Pt 1):G191-200.
USBio References
No references available
Conjugates
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