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You are here:Home » Antibodies » Abs to Mucin » Anti -MUC5AC (Apomucin Major Airway GlycoProtein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin, TBM, Mucin 5AC, Gastric Mucin)

Anti -MUC5AC (Apomucin Major Airway GlycoProtein, Mucin 5 subtype AC tracheobronchial,
Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin,
TBM, Mucin 5AC, Gastric Mucin)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH FC IF
This monoclonal antibody recognizes the peptide core of gastric mucin M1 (>1,000kD) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epi thelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not w ith normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin r eactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fet al colonic) mucins.
Catalog #138572
ApplicationsSuitable for use in ELISA, Flow Cytometry, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.5-1ug/10e6 cells
Western Blot: 0.5-1ug/ml
Immunofluorescence: 1-2ug/ml
Immunoprecipitation: 1-2ug/500ug protein lysate
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT
Optimal dilutions to be determined by the researcher.
Positive ControlStomach
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No45M1
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138572AF.
PurityPurified by Protein G affinity chromatography.
ImmunogenM1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
SpecificityRecognizes human MUC5AC. Species Crossreactivity: feline, chicken, hedgehog, monkey, mouse, porcine, rabbit, rat. Does not react with bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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