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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament (NF, NF-H) (HRP)

Anti -Neurofilament (NF, NF-H) (HRP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH FC
This monoclonal antibody reacts with a 200kD protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kD (NF-L), 160kD (NF-M) and 200kD (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Catalog #138577-HRP
ApplicationsSuitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.5-1ug/10e6 cells
Western Blot: 0.5-1ug/ml
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT
Optimal dilutions to be determined by the researcher.
Positive ControlBrain, Neuroblastoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Clone TypeMonoclonal
IsotypeIgG1
Clone NoNF421
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Conjugated to horseradish peroxidase (HRP).
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to human neurofilament 200kD.
SpecificityRecognizes neurofilament. Species Crossreactivity: human, rat, mouse, porcine and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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