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You are here:Home » Antibodies » Abs to Enzymes, Aminotransferase » Anti -Alanine Aminotransferase (ALT, GPT, ALAT, SGPT, Serum Glutamic Pyruvic Transaminase, Alanine Transaminase)

Anti -Alanine Aminotransferase (ALT, GPT, ALAT, SGPT, Serum Glutamic Pyruvic Transaminase,
Alanine Transaminase)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IC
Catalog #139103
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:100-1:200
Western Blot: 1:100-1:400
Immunohistochemistry (Formalin fixed frozen sections): 1:100-1:500
Immunohistochemistry (Paraffin): 1:50-1:200
Immunocytochemistry (Formalin fixed cells): 1:100-1:500
Optimal dilutions to be determined by the researcher.
Amino Acid SequenceMGHHHHHHSGSEF-PTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL
Positive Control (Included)139103A: Alanine Aminotransferase, Positive Control
Suppled as a recombinant protein (aa367-480) in 100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol.
DAB: 10ul/well
ECL: 5ul/well
Store at -20°C. Stable for 6 months after receipt.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
PurityPurified by affinity chromatography
ImmunogenRecombinant protein corresponding to aa367-480 with a N-Terminal His Tag from human ALT expressed in E. coli.
SpecificityRecognizes human Alanine Aminotransferase (ALT)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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