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You are here:Home » Antibodies » Abs to Peptides » Anti -BNP (Natriuretic Peptides B, Gamma-brain Natriuretic Peptide, NPPB)

Anti -BNP (Natriuretic Peptides B, Gamma-brain Natriuretic Peptide, NPPB)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IC
Catalog #139466
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:100-1:200
Western Blot: 1:100-1:400
Immunohistochemistry (Paraffin): 1:50-1:200
Immunohistochemistry: 1:100-1:500 (formalin fixed frozen sections)
Immunocytochemistry: 1:100-1:500 (formalin fixed cells)
Optimal dilutions to be determined by the researcher.
Amino Acid SequenceMGHHHHHHSGSEF-HPLG SPGSASDLET SGLQEQRNHL QGKLSELQVE QTSLEPLQES PRPTGVWKSR EVATEGIRGH RKMVLYTLRA PRSPKMVQGS GCFGRKMDRI SSSSGLGCKV LRRH
Positive Control Included139466A: BNP, Positive Control
Suppled as a recombinant protein (aa27-137) in 100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol.
DAB: 10ul/well
ECL: 5ul/well
Store at -20°C. Stable for 6 months after receipt.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
PurityPurified by affinity chromatography.
ImmunogenRecombinant protein corresponding to aa27-134 from human BNP with a N-terminal His-tag, expressed in E. coli
SpecificityRecognizes human Brain Natriuretic Peptide (BNP)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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