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You are here:Home » Antibodies » Antibodies-Enzymes, Deoxyribonuclease » Anti -DNASE1 (Deoxyribonuclease-1, Deoxyribonuclease I, DNase I, Dornase alfa, DNL1, DRNI)

Anti -DNASE1 (Deoxyribonuclease-1, Deoxyribonuclease I, DNase I, Dornase alfa, DNL1, DRNI)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IC
Catalog #140049
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:100-1:200
Western Blot: 1:50-1:400
Immunohistochemistry (Formalin fixed frozen sections): 1:50-1:500
Immunohistochemistry (Paraffin): 1:10-1:100
Immunocytochemistry ((Formalin fixed cells) : 1:50-1:500
Optimal dilutions to be determined by the researcher.
Positive Control (Supplied)140049A: Suppled as a recombinant protein (aa19-259) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol).
DAB: 10ul/well
ECL: 5ul/well
Store at -20°C. Stable for 6 months after receipt.
Amino Acid SequenceMGHHHHHHSGSEF-GA VSLKIAAFNI QTFGETKMSN ATLVSYIVQI LSRYDIALVQ EVRDSHLTAV GKLLDNLNQD APDTYHYVVS EPLGRNSYKE RYLFVYRPDQ VSAVDSYYYD DGCEPCGNDT FNREPAIVRF FSRFTEVREF AIVPLHAAPG DAVAEIDALY DVYLDVQEKW GLEDVMLMGD FNAGCSYVRP SQWSSIRLWT SPTFQWLIPD SADTTATPTH CAYDRIVVAG MLLRGAVVPD SALPFNFQA
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
PurityPurified by affinity chromatography.
ImmunogenRecombinant protein corresponding to aa19-259 with two N-Terminal Tags, His-Tag and S-TAG from human DNASE1, expressed in E. coli
SpecificityRecognizes human Deoxyribonuclease I (DNASE1)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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