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You are here:Home » Antibodies » Abs to Hemoglobin » Anti -Glycosylated Hemoglobin (HbA1c) (AP)

Anti -Glycosylated Hemoglobin (HbA1c) (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IC
Catalog #140500-AP
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionELISA: 1:100-200
Western Blot: 1:100-400
Immunocytochemistry: 1:100-500 (formalin fixed cells)
Immunohistochemistry: 1:100-500 (formalin fixed frozen sections)
Immunohistochemistry: 1:50-200 (paraffin sections)
Optimal dilutions to be determined by the researcher.
Included Protein Molecular Weight Marker168631: Unstained Protein Molecular Weight Marker, 10-70kD
Suppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use; no need to heat, dilute or add reducing agents before use.
Protein Bands 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD.
Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier.
Usage Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution. Do not boil! Load the following volumes on SDS-PAGE gel:
Mini gels: 3-5ul/well
Large gel: 7ul/well
The loading volumes listed above are recommended for gels with thickness of 0.75-1mm. The loading volume should be doubled for 1.5mm thick gels.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Note Applications are based on unconjugated antibody.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.05% sodium azide. Labeled with Alkaline Phosphatase (AP).
PurityPurified by affinity chromatography.
ImmunogenNative Protein HbA1c
SpecificityRecognizes human Glycated Hemoglobin (HbA1C)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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