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You are here:Home » Antibodies » Antibodies-IgG4 » Anti -Immunoglobulin G4 (IgG4, IGHG4, Ig Gamma-4 Chain C Region, Immunoglobulin Heavy Constant Gamma 4, G4m Marker, Immunoglobulin Gm4 )

Anti -Immunoglobulin G4 (IgG4, IGHG4, Ig Gamma-4 Chain C Region, Immunoglobulin Heavy
Constant Gamma 4, G4m Marker, Immunoglobulin Gm4 )

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IC
Catalog #140774
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:100-1:200
Western Blot: 1:50-1:400
Immunohistochemistry (Formalin fixed frozen sections): 1:50-1:500
Immunohistochemistry (Paraffin): 1:10-1:100
Immunocytochemistry (Formalin fixed cells): 1:50-1:500
Optimal dilutions to be determined by the researcher.
Amino Acid SequenceMGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QPREPQVYT LPPSQEEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSRL TVDKSRWQEG NVFSCSVMHE ALHNHYTQKS LSLSLGK
Positive Control (Supplied)140774A: Suppled as a recombinant protein (aa222-327) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol).
DAB: 10ul/well
ECL: 5ul/well
Store at -20°C. Stable for 6 months after receipt.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
PurityPurified by affinity chromatography.
ImmunogenRecombinant protein corresponding to aa222-327 with two N-Terminal Tags, His-Tag, T7-Tag from human IgG4, expressed in E. coli
SpecificityRecognizes human Immunoglobulin G4 (IgG4)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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