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You are here:Home » Molecular Biology » Growth Factors-BAFF » BAFF, Recombinant, Human (B Cell Activating Factor, TALL, BLyS, THANK)

BAFF, Recombinant, Human (B Cell Activating Factor, TALL, BLyS, THANK)

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Specifications

Human B cell Activating Factor (BAFF), is a Type II member of the TNF superfamily. Although it is typically a transmembrane protein expressed on T cells, macrophages and dendritic cells, it can also be cleaved in the extracellular region to produce a soluble form detectable in the serum. BAFF is thought to be important for the survival and maturation of peripheral B cells. BAFF initiates signaling through three receptors with human BAFF having activity on mouse splenocytes.
Catalog #142847
SourceRecombinant protein corresponding to 153aa from human BAFF expressed in E.coli.
Molecular Weight~18.5kD
Endotoxin 1EU/ug (LAL)
Biological ActivityThe activity is determined by a RPMI-8226 cell survival assay in the presence of 0.2um DEX and is typically <30ng/ml.
AA SequenceMHHHHHHLVPR MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~18.5kD
SourceE.coli
Purity~90% (HPLC, Reducing and non-reducing SDS-PAGE)
FormSupplied as a lyophilized powder in 10mM Na2PO4, pH 7.5. Reconstitute with sterile dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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