Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Molecular Biology » MB-CD Markers » CD40 Ligand, Recombinant, Human, Animal Free (CD-40-Ligand, AF, TNFSF5, TRAP, CD154, gp39, T-BAM)

CD40 Ligand, Recombinant, Human, Animal Free (CD-40-Ligand, AF, TNFSF5, TRAP, CD154, gp39,
T-BAM)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-y, TNF-a, and IL-2.
Catalog #142854
SourceRecombinant protein corresponding to 149aa from human CD40 Ligand expressed in E.coli.
Molecular Weight:
~16.3kD
Endotoxin 1EU/ug (LAL)
Biological ActivityThe activity is determined by the dose production of IL-8 by human PBMCs and is typically 5-10ng/ml.
AA SequenceMQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~16.3kD
SourceE.coli
Purity~98% (HPLC, Reducing and non-reducing SDS-PAGE)
FormSupplied as a lyophilized powder in 10mM Na2PO4, pH 7.5. Reconstitute with sterile dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links