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You are here:Home » Growth Factors, Cytokines » Growth Factors-FGF » FGF-4, Recombinant, Human (Fibroblast Growth Factor 4, KS3, HBGF-4, HST-1)

FGF-4, Recombinant, Human (Fibroblast Growth Factor 4, KS3, HBGF-4, HST-1)

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Specifications

Fibroblast Growth Factor 4 (FGF-4) is a growth factor predominantly expressed during embryonic development, playing a key role in limb development. In culture, FGF-4 has been shown to be an important regulator of growth for stem cells, fibroblasts and endothelial cells. Unglycosylated FGF-4 is N-terminally cleaved into 13 kDa or 15 kDa proteins that are more active than the precursor 19 kDa protein, Bellosa P, et al. (1993). Human FGF-4 shares high homology and cross- reactivity with the mouse protein.
Catalog #142867
SourceRecombinant protein corresponding to 140aa from human FGF-4 expressed in E.coli.
Molecular Weight~15kD
Endotoxin 1EU/ug (LAL)
Biological ActivityThe activity is determined by its ability to induce the proliferation of mouse NR6R-3T3 fibroblasts and is typically 0.25-1.25ng/ml.
AA SequenceAAVQSGAGDY LLGIKRLRRL YCNVGIGFHL QALPDGRIGG AHADTRDSLL ELSPVERGVV SIFGVASRFF VAMSSKGKLY GSPFFTDECT FKEILLPNNY NAYESYKYPG MFIALSKNGK TKKGNRVSPT MKVTHFLPRL
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~15kD
SourceE.coli
Purity~98% (Reducing and non-reducing SDS-PAGE)
FormSupplied as a lyophilized powder in 0.5 PBS, pH 7.5. Reconstitute with sterile dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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