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You are here:Home » Molecular Biology » Growth Factors-FGF » FGF-basic-154, Recombinant, Human, Animal Free (Fibroblast Growth Factor 154 Basic, AF, FGF2, HBGF-2, Prostatropin)

FGF-basic-154, Recombinant, Human, Animal Free (Fibroblast Growth Factor 154 Basic, AF,
FGF2, HBGF-2, Prostatropin)

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Specifications

Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic 154, also known as FGF-2, is a critical component in keeping embryonic stem cells undifferentiated in cell culture systems.
Catalog #142870
SourceRecombinant protein corresponding to 154aa from human FGF-b 154 expressed in E.coli.
Molecular Weight:
~17.2kD
Endotoxin 1EU/ug (LAL)
Biological ActivityThe activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically <1ng/ml
AA SequenceAAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~17.2kD
SourceE.coli
Purity~97% (Reducing and non-reducing SDS-PAGE)
FormSupplied as a lyophilized powder in 10mM Na2PO4, pH 8.0. Reconstitute with sterile dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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