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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 6, Recombinant, Rat, Animal Free (IL-6, AF, IFN-beta2, B Cell Differentiation Factor, BSF-2, HSF, MGI-2)

Interleukin 6, Recombinant, Rat, Animal Free (IL-6, AF, IFN-beta2, B Cell Differentiation
Factor, BSF-2, HSF, MGI-2)

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Specifications

Interleukin 6 (IL-6) is an important pro-inflammatory and anti-inflammatory cytokine expressed by T cells, macrophages and muscle cells. IL-6 signals through a receptor complex containing two receptors, IL-6Ra and gp130. IL-6 has an important function in promoting fever and can serve to stimulate an immune response to trauma. IL-6 is often used for growth of hybridoma cell lines. Human IL-6 is active on mouse and rat cells.
Catalog #142946
SourceRecombinant protein corresponding to 188aa from rat IL-6 expressed in E.coli.
Molecular Weight~21.8kD
Endotoxin 1EU/ug (LAL)
Biological ActivityThe activity is determined by a dose-dependent stimulation of mouse 7TD1 cells and is typically <0.01ng/ml.
AA SequenceMFPTSQVRRG DFTEDTTHNR PVYTTSQVGG LITYVLREIF EMRKELCNGN SDCMNSDDAL SENNLKLPEI QRNDGCFQTG YNQEICLLKI CSGLLEFRFY LEFVKNNLQD NKKDKARVIQ SNTETLVHIF KQEIKDSYKI VLPTPTSNAL LMEKLESQKE WLRTKTIQLI LKALEEFLKV TMRSTRQT
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~21.8kD
SourceE.coli
Purity~95% (Reducing and non-reducing SDS-PAGE)
FormSupplied as a lyophilized powder in 0.2% TFA, mannitol. Reconstitute with hydrochloride.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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