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You are here:Home » Growth Factors, Cytokines » Growth Factors-PDGF » Platelet Derived Growth Factor AA, Recombinant, Animal Free, Human (AF, PDGF-AA, GDGF, ODGF)

Platelet Derived Growth Factor AA, Recombinant, Animal Free, Human (AF, PDGF-AA, GDGF,
ODGF)

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Specifications

Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-a, TGF-b and EGF.
Catalog #142965
SourceRecombinant protein corresponding to two 125aa chains from human PDGF- AA expressed in E.coli.
Molecular Weight~28.5kD
Endotoxin 1EU/ug (LAL)
Biological ActivityThe activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically 3-5ng/ml.
AA SequenceSIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity~97% (Reducing and non-reducing SDS-PAGE)
FormSupplied as a lyophilized powder. No additives. Reconstitute with sterile dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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