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You are here:Home » Molecular Biology » Growth Factors-PDGF » Platelet Derived Growth Factor AA, Recombinant, Mouse (PDGF-AA, GDGF, ODGF)

Platelet Derived Growth Factor AA, Recombinant, Mouse (PDGF-AA, GDGF, ODGF)

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Specifications

Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-a, TGF-b and EGF.
Catalog #142966
SourceRecombinant protein corresponding to two 126aa chains mouse PDGF-AA expressed in E.coli.
Molecular Weight~28.9kD
Endotoxin 1EU/ug (LAL)
Biological ActivityThe activity is determined by the dose-dependent proliferation of Balb/c 3T3 cells and is typically 8-10ng/ml.
AA SequenceMSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~28.9kD
SourceE.coli
Purity~97% (Reducing and non-reducing SDS-PAGE)
FormSupplied as a lyophilized powder. No additives. Reconstitute with sterile dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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