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You are here:Home » Growth Factors, Cytokines » Growth Factors-PDGF » Platelet Derived Growth Factor BB, Recombinant, Mouse (PDGF-BB, GDGF, ODGF)

Platelet Derived Growth Factor BB, Recombinant, Mouse (PDGF-BB, GDGF, ODGF)

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Specifications

Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-a, TGF-b and EGF.-b
Catalog #142969
SourceRecombinant protein corresponding to two 109aa chains mouse PDGF-BB expressed in E.coli.
Molecular Weight~24.6kD
Endotoxin 1EU/ug (LAL)
Biological ActivityThe activity is measured by the dose-dependent proliferation in mouse 3T3 fibroblasts and is typically 1-2ng/ml.
AA SequenceMSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~24.6kD
SourceE.coli
Purity~98% (Reducing and non-reducing SDS-PAGE)
FormSupplied as a lyophilized powder in 10mM sodium citrate, pH 3.0. Reconstitute with sterile dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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