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You are here:Home » Molecular Biology » MB-Sonic Hedgehog (SHH) » SHH, Recombinant, Human (Sonic Hedgehog, HHG-1)

SHH, Recombinant, Human (Sonic Hedgehog, HHG-1)

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Specifications

Sonic hedgehog (SHH) is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology and all signal via the same receptors.
Catalog #142979
SourceRecombinant protein corresponding to 175aa from human SHH expressed in E.coli.
Molecular Weight~19.7kD
Endotoxin 1EU/ug (LAL)
Biological ActivityThe activity is measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 (CCL-226) fibroblasts and is typically 0.8-1ug/ml.
AA SequenceMIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~19.7 kD
SourceE.coli
Purity~97% (Reducing and non-reducing SDS-PAGE)
FormSupplied as a lyophilized powder in 10mM Na2PO4, pH 7.5. Reconstitute with sterile dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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